Lyme disease testing follows a two-tier approach: an initial screening immunoassay (such as ELISA), followed by Western blot confirmation of positive or equivocal results. The Western blot detects antibodies to specific Borrelia proteins, with characteristic patterns of band positivity used to diagnose past or current Lyme disease.
This test is used as confirmation following positive Lyme screening tests in patients with relevant exposure or symptoms. Interpretation depends on which specific bands are positive and whether IgM or IgG antibodies are tested. Sample type is serum.
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