This test measures the concentration of 25-hydroxyvitamin D — the main circulating form of vitamin D and the best marker of overall vitamin D stores. LC/MS/MS technology provides superior accuracy by directly measuring both D2 and D3 forms, eliminating the cross-reactivity issues seen with immunoassay methods.
Clinicians order this test to assess deficiency in patients with bone disorders, malabsorption conditions, chronic kidney disease, autoimmune conditions, or nonspecific symptoms such as fatigue and muscle weakness. It is also valuable for monitoring patients on vitamin D supplementation. The sample is serum, and fasting is not typically required.
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